{"id":360,"date":"2017-05-19T23:37:57","date_gmt":"2017-05-19T14:37:57","guid":{"rendered":"https:\/\/amphibian.hiroshima-u.ac.jp\/?page_id=360"},"modified":"2019-10-17T20:41:03","modified_gmt":"2019-10-17T11:41:03","slug":"bioresource","status":"publish","type":"page","link":"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/bioresource","title":{"rendered":"\u7814\u7a76\u5185\u5bb9"},"content":{"rendered":"<div id=\"pl-360\" class=\"panel-layout\">\n<div id=\"pg-360-0\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-360-0-0\" class=\"panel-grid-cell\" data-weight=\"1\">&nbsp;<\/div>\n<\/div>\n<div id=\"pg-360-2\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;,&quot;iw-visible-screen&quot;:&quot;iw-all&quot;,&quot;iw-visible-layout&quot;:&quot;iw-all&quot;}\" data-ratio=\"1\" data-ratio-direction=\"right\">\n<div id=\"pgc-360-2-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-360-2-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"3\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;iw-visible-screen&quot;:&quot;iw-all&quot;,&quot;iw-visible-layout&quot;:&quot;iw-all&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<h3 id=\"gaiyou\">1. \u7814\u7a76\u8ab2\u984c<\/h3>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1018 alignright\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2017\/05\/31297bd32b139da1702201cfac006fb8.jpg\" alt=\"\" width=\"315\" height=\"211\" srcset=\"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/31297bd32b139da1702201cfac006fb8.jpg 3300w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/31297bd32b139da1702201cfac006fb8-300x201.jpg 300w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/31297bd32b139da1702201cfac006fb8-768x514.jpg 768w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/31297bd32b139da1702201cfac006fb8-1024x686.jpg 1024w\" sizes=\"auto, (max-width: 315px) 100vw, 315px\" \/>\u3000\u672c\u90e8\u9580\u3067\u306f\u3001\u767a\u751f\u3084\u518d\u751f\u3001\u9032\u5316\u306e\u4ed5\u7d44\u307f\u3092\u3001\u30b2\u30ce\u30e0\u30fb\u30a8\u30d4\u30b2\u30ce\u30e0\u5236\u5fa1\u306e\u5074\u9762\u304b\u3089\u89e3\u660e\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u3066\u3001\u4e0b\u8a18\u306e\u7814\u7a76\u8ab2\u984c\uff11\uff09\u301c\uff13\uff09\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3059\u3002\u7814\u7a76\u6750\u6599\u306b\u306f\u3001\u4e3b\u306b\uff12\u7a2e\u985e\u306e\u30c4\u30e1\u30ac\u30a8\u30eb\uff08\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u3068\u30cd\u30c3\u30bf\u30a4\u30c4\u30e1\u30ac\u30a8\u30eb\uff09\u3068\u30ea\u30e5\u30a6\u30ad\u30e5\u30a6\u30ab\u30b8\u30ab\u30ac\u30a8\u30eb\u3092\u7528\u3044\u3066\u3044\u307e\u3059\u3002\u90e8\u9580\u9577\u306f\u30cd\u30c3\u30bf\u30a4\u30c4\u30e1\u30ac\u30a8\u30eb\u3092\u7528\u3044\u305f\u5b9f\u9a13\u7cfb\u306e\u958b\u767a\u8005\u3067\u3042\u308a\u3001\u9ad8\u52b9\u7387\u306a\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cd\u30b7\u30b9\u6280\u8853\u306e\u958b\u767a\u3084\uff08I-SceI\u6cd5\u3001<strong><em>Nature Protocol<\/em><\/strong> 1: 1703-1710 (2006)\u3001<strong><em>Dev. Growth Differ.<\/em><\/strong> 51: 387-401 (2009)\uff09\u3001\u5168\u30b2\u30ce\u30e0\u914d\u5217\u306e\u89e3\u8aad\uff08<strong>\u6bce\u65e5\u65b0\u805e\u3001\u65e5\u672c\u7d4c\u6e08\u65b0\u805e\u4ed6\u3067\u5831\u9053\u3001<\/strong><strong><em>Science<\/em><\/strong> 328: 633-636 (2010)\uff09\u7b49\u306b\u643a\u308f\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u5b9f\u9a13\u306b\u306f\u3001\u3053\u308c\u3089\u306e\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cd\u30b7\u30b9\u6280\u8853\u3084\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u305f\u751f\u4f53\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3092\u7528\u3044\u305f\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30c8\u30fc\u30e0\u89e3\u6790\u3001\u30af\u30ed\u30de\u30c1\u30f3\u514d\u75ab\u6c88\u964d\u3001\u6bd4\u8f03\u30b2\u30ce\u30e0\u89e3\u6790\u7b49\u3001\u30a6\u30a7\u30c3\u30c8\u304b\u3089\u30c9\u30e9\u30a4\u307e\u3067\u69d8\u3005\u306a\u5148\u92ed\u7684\u65b9\u6cd5\u3092\u99c6\u4f7f\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h5 id=\"kadai1\">1) \u30b2\u30ce\u30e0\u91cd\u8907\u306b\u4f34\u3046\u907a\u4f1d\u5b50\u9032\u5316\u306e\u7814\u7a76<\/h5>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1021 alignright\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2017\/05\/4fa30f19409ebd47f0ec13167a31735f.jpg\" alt=\"\" width=\"407\" height=\"273\" srcset=\"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/4fa30f19409ebd47f0ec13167a31735f.jpg 491w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/4fa30f19409ebd47f0ec13167a31735f-300x201.jpg 300w\" sizes=\"auto, (max-width: 407px) 100vw, 407px\" \/>\u3000\u3053\u308c\u307e\u3067\u306e\u7814\u7a76\u304b\u3089\u3001\uff15\u5104\u5e74\u4ee5\u4e0a\u524d\u306e\u30ab\u30f3\u30d6\u30ea\u30a2\u7d00\u306b\u3001\u810a\u690e\u52d5\u7269\u306e\u7956\u5148\u7a2e\u306b\u304a\u3044\u3066\u3001\u5168\u3066\u306e\u907a\u4f1d\u5b50\u306e\u30b3\u30d4\u30fc\u6570\u304c\u500d\u306b\u5897\u3048\u308b\u300c\u5168\u30b2\u30ce\u30e0\u91cd\u8907\u300d\u3068\u3088\u3070\u308c\u308b\u73fe\u8c61\u304c\u8d77\u304d\u305f\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u4f59\u5270\u306a\u907a\u4f1d\u5b50\u304c\u751f\u307e\u308c\u3001\u305d\u306e\u767a\u73fe\u30d1\u30bf\u30fc\u30f3\u304c\u591a\u69d8\u5316\u3057\u305f\u7d50\u679c\u3001\u3088\u308a\u8907\u96d1\u306a\u4f53\u304c\u9032\u5316\u3057\u305f\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u5177\u4f53\u7684\u306b\u306f\u3001\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u8abf\u7bc0\u914d\u5217\uff08\u30b7\u30b9\u8abf\u7bc0\u914d\u5217\uff09\u306e\u3069\u306e\u3088\u3046\u306a\u5909\u5316\u306b\u3088\u3063\u3066\u3001\u3053\u306e\u767a\u73fe\u30d1\u30bf\u30fc\u30f3\u306e\u5909\u5316\u304c\u5f15\u304d\u8d77\u3053\u3055\u308c\u305f\u306e\u304b\u3001\u4e0d\u660e\u306a\u307e\u307e\u3067\u3057\u305f\u3002\u3053\u306e\u554f\u984c\u306b\u5bfe\u3057\u3066\u79c1\u9054\u306f\u3001\u773c\u3084\u8133\u3001\u814e\u81d3\u306e\u5f62\u6210\u3092\u5236\u5fa1\u3059\u308b<em>Pax<\/em>\u907a\u4f1d\u5b50\u30d5\u30a1\u30df\u30ea\u30fc\u306b\u6ce8\u76ee\u3057\u3001\u305d\u306e\u767a\u73fe\u8abf\u7bc0\u306e\u4ed5\u7d44\u307f\u3092\u30c4\u30e1\u30ac\u30a8\u30eb\u3001\u30de\u30a6\u30b9\u3001\u305d\u3057\u3066\u810a\u690e\u52d5\u7269\u306e\u7956\u5148\u7a2e\u306b\u8fd1\u7e01\u3067\u300c\u751f\u304d\u305f\u5316\u77f3\u300d\u3068\u3088\u3070\u308c\u308b\u30ca\u30e1\u30af\u30b8\u30a6\u30aa\u3068\u306e\u9593\u3067\u3001\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u5b9f\u9a13\u306b\u3088\u308a\u6bd4\u8f03\u89e3\u6790\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u8abf\u7bc0\u914d\u5217\u306e\u3046\u3061\u6d3b\u6027\u5316\u306b\u50cd\u304f\u90e8\u5206\uff08\u30a8\u30f3\u30cf\u30f3\u30b5\u30fc\uff09\u306f\u3001\u91cd\u8907\u907a\u4f1d\u5b50\u306e\u9593\u3067\u3042\u307e\u308a\u5909\u5316\u3057\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u3001\u3053\u306e\u30a8\u30f3\u30cf\u30f3\u30b5\u30fc\u306e\u4f5c\u7528\u3092\u90e8\u5206\u7684\u306b\u6253\u3061\u6d88\u3059\u914d\u5217\uff08\u30b5\u30a4\u30ec\u30f3\u30b5\u30fc\uff09\u304c\u3001\u91cd\u8907\u907a\u4f1d\u5b50\u306e\u7247\u65b9\u306b\u65b0\u305f\u306b\u4ed8\u52a0\u3055\u308c\u3001\u767a\u73fe\u30d1\u30bf\u30fc\u30f3\u304c\u5927\u304d\u304f\u5909\u5316\u3057\u305f\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u304a\u304a\u307e\u304b\u306a\u4e0b\u7d75\u304c\u3001\u30a8\u30f3\u30cf\u30f3\u30b5\u30fc\u3088\u3063\u3066\uff15\u5104\u5e74\u4ee5\u4e0a\u3082\u6614\u306e\u7956\u5148\u7a2e\u3067\u65e2\u306b\u5b8c\u6210\u3057\u3066\u3044\u305f\u3053\u3068\u3001\u305d\u3057\u3066\u5168\u30b2\u30ce\u30e0\u91cd\u8907\u306b\u3088\u308a\u907a\u4f1d\u5b50\u306e\u30b3\u30d4\u30fc\u6570\u304c\u5897\u3048\u305f\u5f8c\u306b\u3001\u5897\u3048\u305f\u907a\u4f1d\u5b50\u304c\u5fc5\u8981\u306a\u5834\u6240\u3060\u3051\u3067\u50cd\u304d\u3001\u4f59\u8a08\u306a\u5834\u6240\u3067\u306f\u50cd\u304b\u306a\u3044\u3088\u3046\u306b\u3001\u30b5\u30a4\u30ec\u30f3\u30b5\u30fc\u304c\u4ed8\u3051\u52a0\u308f\u3063\u3066\u3044\u3063\u305f\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\uff08<strong>\u6bce\u65e5\u65b0\u805e\u3001\u7523\u7d93\u65b0\u805e\u4ed6\u3067\u5831\u9053\u3001<\/strong><strong><em>Nature Communications<\/em><\/strong> 3: 848 (2012)\uff09\u3002<br><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1023 alignright\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2017\/05\/3178dcd4b61707985eb2650b4082162c.jpg\" alt=\"\" width=\"407\" height=\"237\" srcset=\"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/3178dcd4b61707985eb2650b4082162c.jpg 532w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/3178dcd4b61707985eb2650b4082162c-300x175.jpg 300w\" sizes=\"auto, (max-width: 407px) 100vw, 407px\" \/>\u3000\u307e\u305f\u3001\u810a\u690e\u52d5\u7269\u306e\u30b0\u30eb\u30fc\u30d7\u306b\u304a\u3044\u3066\u3001\u30b2\u30ce\u30e0\u91cd\u8907\u306f\u305d\u306e\u7956\u5148\u7a2e\u306e\u307f\u306a\u3089\u305a\u3001\u305d\u306e\u5f8c\u3082\u69d8\u3005\u306a\u7a2e\u306e\u5206\u5c90\u904e\u7a0b\u3067\u8d77\u304d\u305f\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u4e21\u751f\u985e\u3067\u306f\u3001\u30cd\u30c3\u30bf\u30a4\u30c4\u30e1\u30ac\u30a8\u30eb\u3068\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u306e\u7956\u5148\u7a2e\u304c\u5206\u5c90\u3057\u305f\u5f8c\u3001\u308f\u305a\u304b1700\u4e07\u5e74\u307b\u3069\u524d\u306b\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u306e\u7cfb\u8b5c\u3067\u5168\u30b2\u30ce\u30e0\u91cd\u8907\u304c\u8d77\u304d\u307e\u3057\u305f\u3002\u79c1\u9054\u306f\u30b2\u30ce\u30e0\u91cd\u8907\u5f8c\u306e\u907a\u4f1d\u5b50\u9032\u5316\u306e\u59cb\u307e\u308a\u3068\u306f\u3069\u306e\u3088\u3046\u306a\u3082\u306e\u304b\u3092\u89e3\u660e\u3059\u308b\u70ba\u3001<em>Six6<\/em>\u3068\u3088\u3070\u308c\u308b\u773c\u5f62\u6210\u907a\u4f1d\u5b50\u306b\u6ce8\u76ee\u3057\u3001\u30cd\u30c3\u30bf\u30a4\u30c4\u30e1\u30ac\u30a8\u30eb\u304c\uff11\u3064\u3060\u3051\u3082\u3064\u7956\u5148\u578b\u3068\u3001\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u3067\uff12\u3064\u306b\u5897\u3048\u305f\u30b3\u30d4\u30fc\u306b\u3064\u3044\u3066\u6bd4\u8f03\u7814\u7a76\u3092\u304a\u3053\u306a\u3044\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u306e\uff12\u3064\u306e<em>Six6<\/em>\u907a\u4f1d\u5b50\u306e\u3046\u3061\u3001\uff11\u3064\u306e\u30b3\u30d4\u30fc\u306e\u30a8\u30f3\u30cf\u30f3\u30b5\u30fc\u306b\u5909\u7570\u304c\u84c4\u7a4d\u3057\u3066\u767a\u73fe\u91cf\u304c\u4f4e\u4e0b\u3057\u3001\u305d\u308c\u306b\u4f34\u3063\u3066\u540c\u3058\u30b3\u30d4\u30fc\u306e\u30b3\u30fc\u30c9\u914d\u5217\u306b\u3082\u30a2\u30df\u30ce\u9178\u7f6e\u63db\u3092\u4f34\u3046\u5909\u7570\u304c\u84c4\u7a4d\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u3053\u3068\u306f\u3001\u540c\u3058\u6a5f\u80fd\u3092\u3082\u3064\u907a\u4f1d\u5b50\u304c\uff12\u3064\u3067\u304d\u305f\u5f8c\u306b\u3001\u4e00\u65e6\u7247\u65b9\u306e\u30b3\u30d4\u30fc\u306e\u767a\u73fe\u91cf\u304c\u4f4e\u4e0b\u3059\u308b\u3068\u305d\u306e\u30b3\u30d4\u30fc\u306b\u5bfe\u3059\u308b\u9078\u629e\u5727\u304c\u4f4e\u4e0b\u3057\u3001\u30b3\u30fc\u30c9\u914d\u5217\u306b\u3082\u5909\u7570\u304c\u84c4\u7a4d\u3057\u6613\u304f\u306a\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\uff08<strong><em>Dev. Biol.<\/em><\/strong>, 427: 84-92 (2017)\uff09\u3002\u307e\u305f\u8208\u5473\u6df1\u3044\u3053\u3068\u306b\u3001\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u306e<em>Six6<\/em>\u907a\u4f1d\u5b50\u306e\uff11\u3064\u306b\u898b\u3064\u304b\u3063\u305f\u30a2\u30df\u30ce\u9178\u7f6e\u63db\u5909\u7570\u3068\u985e\u4f3c\u3057\u305f\u5909\u7570\u304c\u3001\u30d2\u30c8\u306e\u5c0f\u773c\u75c7\u60a3\u8005\u306b\u898b\u3064\u304b\u3063\u3066\u3044\u307e\u3059\u3002\u73fe\u5728\u306f\u3001\u9032\u5316\u306b\u304a\u3051\u308b\u907a\u4f1d\u5b50\u5909\u7570\u3068\u3001\u75be\u60a3\u306e\u539f\u56e0\u3068\u306a\u308b\u907a\u4f1d\u5b50\u5909\u7570\u3068\u306e\u76f8\u95a2\u95a2\u4fc2\u306b\u3064\u3044\u3066\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n<h5>2) \u773c\u306e\u767a\u751f\u9032\u5316\u306b\u304a\u3051\u308b\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u5236\u5fa1\u306e\u7814\u7a76<\/h5>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1022 alignright\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2017\/05\/8b3ac198ebb4c75b99c045ff837a995b.jpg\" alt=\"\" width=\"377\" height=\"283\" srcset=\"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/8b3ac198ebb4c75b99c045ff837a995b.jpg 513w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/8b3ac198ebb4c75b99c045ff837a995b-300x225.jpg 300w\" sizes=\"auto, (max-width: 377px) 100vw, 377px\" \/>\u8fd1\u5e74\u3001\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u8abf\u7bc0\u306b\u304a\u3051\u308b\u30af\u30ed\u30de\u30c1\u30f3\u69cb\u9020\u5236\u5fa1\u306e\u91cd\u8981\u6027\u304c\u3001\u69d8\u3005\u306a\u7814\u7a76\u304b\u3089\u660e\u3089\u304b\u306b\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u30af\u30ed\u30de\u30c1\u30f3\u306e\u90e8\u4f4d\u7279\u7570\u7684\u306a\u51dd\u7e2e\u3068\u5f1b\u7de9\u306f\u3001\u305d\u306e\u90e8\u4f4d\u306b\u542b\u307e\u308c\u308b\u907a\u4f1d\u5b50\u306e\u8ee2\u5199\u6291\u5236\u3068\u6d3b\u6027\u5316\u306b\u50cd\u304d\u307e\u3059\u304c\u3001\u305d\u308c\u3089\u306e\u5236\u5fa1\u306b\u306f\u30d2\u30b9\u30c8\u30f3\u306e\u30e1\u30c1\u30eb\u5316\u3084\u30a2\u30bb\u30c1\u30eb\u5316\u304c\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u3053\u308c\u3089\u907a\u4f1d\u5b50\u3054\u3068\u306e\u30e1\u30c1\u30eb\u5316\u4fee\u98fe\u3084\u30a2\u30bb\u30c1\u30eb\u5316\u4fee\u98fe\u306e\u30d1\u30bf\u30fc\u30f3\u306f\u3001\u7d30\u80de\u5206\u88c2\u3092\u7d4c\u3066\u5a18\u7d30\u80de\u306b\u5f15\u304d\u7d99\u304c\u308c\u308b\u70ba\u3001\u3044\u308f\u3070\u7d30\u80de\u8a18\u61b6\u3068\u3057\u3066\u50cd\u304d\u307e\u3059\u3002\u79c1\u9054\u306f\u3053\u308c\u3089\u306e\u30d2\u30b9\u30c8\u30f3\u4fee\u98fe\u306e\u4e2d\u3067\u3082\u3001\u7279\u306b\u30af\u30ed\u30de\u30c1\u30f3\u51dd\u7e2e\u306b\u4f5c\u7528\u3059\u308b\u30d2\u30b9\u30c8\u30f3H3K27\u30e1\u30c1\u30eb\u5316\u56e0\u5b50\u7fa4\uff08\u30dd\u30ea\u30b3\u30fc\u30e0PRC2\u8907\u5408\u4f53\u69cb\u6210\u56e0\u5b50\u7fa4\uff09\u3068\u3001\u305d\u308c\u3089\u306b\u62ee\u6297\u3059\u308b\u8131\u30e1\u30c1\u30eb\u5316\u56e0\u5b50\u7fa4\u306b\u6ce8\u76ee\u3057\u3066\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u3001\u767a\u751f\u306b\u304a\u3044\u3066\u306f\u3001H3K27\u8131\u30e1\u30c1\u30eb\u5316\u56e0\u5b50\u304c\u611f\u899a\u5668\u30d7\u30e9\u30b3\u30fc\u30c9\uff08\u773c\u306e\u30ec\u30f3\u30ba\u3084\u9f3b\u306e\u55c5\u4e0a\u76ae\u3001\u8033\u306e\u5185\u8033\u7b49\u306e\u611f\u899a\u5668\u306e\u5171\u901a\u539f\u57fa\uff09\u3067\u767a\u73fe\u3057\u3001\u3053\u306e\u611f\u899a\u5668\u30d7\u30e9\u30b3\u30fc\u30c9\u306e\u4e00\u90e8\u304b\u3089\u30ec\u30f3\u30ba\u304c\u5206\u5316\u3059\u308b\u306e\u306b\u5fc5\u8981\u306a\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u611f\u899a\u5668\u306f\u3001\u30ca\u30e1\u30af\u30b8\u30a6\u30aa\u306e\u3088\u3046\u306a\u539f\u59cb\u7684\u306a\u810a\u7d22\u52d5\u7269\u304b\u3089\u810a\u690e\u52d5\u7269\u304c\u9032\u5316\u3059\u308b\u904e\u7a0b\u3067\u7372\u5f97\u3055\u308c\u305f\u5668\u5b98\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u3086\u3048\u3001\u773c\u306e\u9032\u5316\u306b\u304a\u3051\u308bH3K27\u8131\u30e1\u30c1\u30eb\u5316\u56e0\u5b50\u306e\u5f79\u5272\u306b\u3064\u3044\u3066\u3082\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n<h5 id=\"kadai3\">3) \u810a\u9ac4\u518d\u751f\u306b\u304a\u3051\u308b\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u5236\u5fa1\u306e\u7814\u7a76<\/h5>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1024 alignright\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2017\/05\/10ab2843fbcd1dc2acb1f3fb90a9f4df.jpg\" alt=\"\" width=\"369\" height=\"111\" srcset=\"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/10ab2843fbcd1dc2acb1f3fb90a9f4df.jpg 545w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/10ab2843fbcd1dc2acb1f3fb90a9f4df-300x90.jpg 300w\" sizes=\"auto, (max-width: 369px) 100vw, 369px\" \/>\u3000\u79c1\u9054\u30d2\u30c8\u3092\u542b\u3080\u54fa\u4e73\u985e\u306f\u3001\u4e8b\u6545\u306a\u3069\u3067\u640d\u50b7\u3057\u305f\u810a\u9ac4\u3092\u5341\u5206\u306b\u518d\u751f\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u30c4\u30e1\u30ac\u30a8\u30eb\u5e7c\u751f\u306f\u3001\u810a\u9ac4\u3092\u542b\u3080\u5c3e\u90e8\u3092\u5207\u65ad\u3057\u3066\u3082\uff12\u9031\u9593\u307b\u3069\u3067\u518d\u751f\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u518d\u751f\u904e\u7a0b\u306f\u69d8\u3005\u306a\u7a2e\u985e\u306e\u5e79\u7d30\u80de\u306e\u5897\u6b96\u3068\u5206\u5316\u3092\u4f34\u3044\u3001\u305d\u3053\u3067\u306f\u975e\u5e38\u306b\u591a\u304f\u306e\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u304c\u6291\u5236\u3042\u308b\u3044\u306f\u6d3b\u6027\u5316\u3055\u308c\u307e\u3059\u3002\u79c1\u9054\u306f\u3001\u3053\u306e\u30b2\u30ce\u30e0\u30ef\u30a4\u30c9\u306a\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u5207\u66ff\u3048\u306bH3K27\u30e1\u30c1\u30eb\u5316\u56e0\u5b50\u7fa4\u3068\u305d\u308c\u3089\u306b\u62ee\u6297\u8131\u30e1\u30c1\u30eb\u5316\u7fa4\u306e\u4e21\u65b9\u304c\u5fc5\u8981\u306a\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u73fe\u5728\u3001\u305d\u308c\u3089\u306e\u30e1\u30c1\u30eb\u5316\u5236\u5fa1\u56e0\u5b50\u7fa4\u306e\u6a19\u7684\u907a\u4f1d\u5b50\u306e\u63a2\u7d22\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n<h5 id=\"kadai4\">4) \u751f\u7269\u306e\u74b0\u5883\u9069\u5fdc\u306b\u304a\u3051\u308b\u907a\u4f1d\u5b50\u9032\u5316\u30fb\u30b2\u30ce\u30e0\u9032\u5316\u306e\u7814\u7a76<\/h5>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1382 alignright\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2017\/11\/78692eb8438a92f7e11a516981ad7fad.png\" alt=\"\" width=\"401\" height=\"267\" srcset=\"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/11\/78692eb8438a92f7e11a516981ad7fad.png 959w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/11\/78692eb8438a92f7e11a516981ad7fad-300x200.png 300w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/11\/78692eb8438a92f7e11a516981ad7fad-768x513.png 768w\" sizes=\"auto, (max-width: 401px) 100vw, 401px\" \/>\u3000\u73fe\u5728\u5730\u7403\u4e0a\u306b\u5b58\u5728\u3059\u308b\u3059\u3079\u3066\u306e\u751f\u7269\u306f\u3001\u305d\u308c\u305e\u308c\u306e\u751f\u606f\u74b0\u5883\u306b\u9069\u5fdc\u3059\u308b\u3053\u3068\u3067\u751f\u5b58\u3057\u3001\u591a\u69d8\u306a\u7a2e\u751f\u614b\u3092\u6301\u3064\u306b\u81f3\u3063\u3066\u3044\u307e\u3059\u3002\u305d\u3046\u3057\u305f\u69d8\u3005\u306a\u751f\u7269\u306e\u7279\u5fb4\u306f\u3069\u306e\u3088\u3046\u306a\u907a\u4f1d\u5b50\u3084\u30b2\u30ce\u30e0\u69cb\u9020\u306e\u5909\u5316\u306b\u3088\u3063\u3066\u3082\u305f\u3089\u3055\u308c\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u7279\u306b\u3001\u6e29\u5ea6\u3078\u306e\u9069\u5fdc\u306f\u3059\u3079\u3066\u306e\u751f\u7269\u304c\u907f\u3051\u3089\u308c\u306a\u3044\u74b0\u5883\u8981\u7d20\u3067\u3059\u3002\u307e\u305f\u3001\u6211\u3005\u30d2\u30c8\u3092\u306f\u3058\u3081\u3068\u3059\u308b\u6052\u6e29\u751f\u7269\u306f\u6e29\u5ea6\u3092\u4e00\u5b9a\u306b\u4fdd\u3064\u3053\u3068\u3067\u5206\u5e03\u3092\u5e83\u3052\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u3082\u305d\u3082\u3069\u306e\u3088\u3046\u306b\u3057\u306637\u2103\u3068\u3044\u3046\u9ad8\u6e29\u306b\u9069\u5fdc\u3057\u305f\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u79c1\u9054\u306f\u3001\u7409\u7403\u5217\u5cf6\u306b\u751f\u606f\u3059\u308b\u30ea\u30e5\u30a6\u30ad\u30e5\u30a6\u30ab\u30b8\u30ab\u30ac\u30a8\u30eb\u304c\u9ad8\u6e29\u8010\u6027\u3092\u7372\u5f97\u3057\u3001\u5e7c\u751f\u304c40\u2103\u3092\u8d8a\u3048\u308b\u6e29\u6cc9\u306b\u751f\u606f\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u4e21\u751f\u985e\u304c\u751f\u606f\u3059\u308b\u6c34\u6e29\u306e\u4e16\u754c\u6700\u9ad8\u8a18\u9332\u3067\u3059\u3002\u79c1\u9054\u306f\u3053\u306e\u30ea\u30e5\u30a6\u30ad\u30e5\u30a6\u30ab\u30b8\u30ab\u30ac\u30a8\u30eb\u3068\u305d\u306e\u8fd1\u7e01\u7a2e\u306b\u3064\u3044\u3066\u3001\u9ad8\u6e29\u8010\u6027\u95a2\u9023\u907a\u4f1d\u5b50\u306e\u5358\u96e2\u3068\u6a5f\u80fd\u89e3\u6790\u3092\u884c\u3046\u3068\u3068\u3082\u306b\u3001\u5168\u30b2\u30ce\u30e0\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u8aad\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n<h5 id=\"jyohi5\">5) \u4e0a\u76ae\u6027\u5668\u5b98\u306e\u5f62\u6210\u6a5f\u69cb\u3068\u30d2\u30c8\u5148\u5929\u7570\u5e38\u306b\u95a2\u3059\u308b\u7814\u7a76<\/h5>\n<div id=\"attachment_3227\" style=\"width: 310px\" class=\"wp-caption alignright fbx-instance\"><a class=\"fbx-link\" href=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2019\/07\/Fig1_600dpi.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-3227\" class=\"size-medium wp-image-3227\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2019\/07\/Fig1_600dpi-300x159.jpg\" alt=\"\u56f31 \u30c4\u30e1\u30ac\u30a8\u30eb\u306b\u304a\u3051\u308b\u795e\u7d4c\u7ba1\u306e\u5f62\u6210\u904e\u7a0b\" width=\"300\" height=\"159\" srcset=\"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2019\/07\/Fig1_600dpi-300x159.jpg 300w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2019\/07\/Fig1_600dpi-768x407.jpg 768w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2019\/07\/Fig1_600dpi-1024x543.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-3227\" class=\"wp-caption-text\">\u56f31 \u30c4\u30e1\u30ac\u30a8\u30eb\u306b\u304a\u3051\u308b\u795e\u7d4c\u7ba1\u306e\u5f62\u6210\u904e\u7a0b<br>Suzuki et al., Dev. Growth Differ., 54, 266-276, 2012\u3088\u308a<\/p><\/div>\n<p>\u3000\u4e0a\u76ae\u6027\u5668\u5b98\u306f\u4e0a\u76ae\u7d30\u80de\u306e\u96c6\u56e3\u304c\u7ba1\u72b6\u3084\u51f9\u51f8\u72b6\u306e\u7acb\u4f53\u69cb\u9020\u3092\u53d6\u308b\u3053\u3068\u3067\u5f62\u6210\u3055\u308c\u308b\u52d5\u7269\u306e\u57fa\u672c\u7684\u306a\u751f\u4f53\u69cb\u9020\u3067\u3059\u3002\u306a\u304b\u3067\u3082\u3001\u308f\u305f\u3057\u305f\u3061\u306e\u8133\u3084\u810a\u9ac4\u306e\u3082\u3068\u306b\u306a\u308b\u795e\u7d4c\u7ba1\u306f\u5e73\u9762\u306e\u7d30\u80de\u30b7\u30fc\u30c8\u304b\u3089\u7ba1\u3092\u3064\u304f\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u5f62\u3065\u304f\u3089\u308c\u307e\u3059\uff08\u56f3\uff11\uff09\u3002\u3053\u306e\u795e\u7d4c\u7ba1\u304c\u5f62\u6210\u3055\u308c\u308b\u904e\u7a0b\uff08\u795e\u7d4c\u7ba1\u5f62\u6210\uff09\u3067\u306f\u7d30\u80de\u306e\u5f62\u614b\u304c\u7d30\u9577\u304f\u5909\u5316\u3057\uff08\u7d30\u80de\u4f38\u9577\uff09\u3001\u52a0\u3048\u3066\u304f\u3055\u3073\u578b\u3078\u5909\u5316\u3059\u308b\uff08\u9802\u7aef\u53ce\u7e2e\uff09\u3053\u3068\u304c\u91cd\u8981\u3067\u3059 (\u56f3\uff12\u3001Dev. Growth Differ., 54, 266-276, 2012)\u3002<span style=\"font-size: inherit;\">\u79c1\u9054\u306f\u30c4\u30e1\u30ac\u30a8\u30eb\u306e\u795e\u7d4c\u7ba1\u3092\u30e2\u30c7\u30eb\u3068\u3057\u305f\u7d30\u80de\u751f\u7269\u5b66\u7684\u306a\u89e3\u6790\u306b\u3088\u308a\u3001\u4e0a\u76ae\u6027\u5668\u5b98\u306e\u5f62\u6210\u306b\u95a2\u308f\u308b\u7d30\u80de\u30fb\u7d30\u80de\u9aa8\u683c\u306e\u52d5\u614b\u3084\u305d\u306e\u5236\u5fa1\u6a5f\u69cb\u3092\u660e\u3089\u304b\u306b\u3057\u3088\u3046\u3068\u3057\u3066\u3044\u307e\u3059\u3002<\/span><\/p>\n<div id=\"attachment_3228\" style=\"width: 310px\" class=\"wp-caption alignright fbx-instance\"><a class=\"fbx-link\" href=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2019\/07\/Fig2_600dpi.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-3228\" class=\"size-medium wp-image-3228\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2019\/07\/Fig2_600dpi-300x201.jpg\" alt=\"\u56f3\uff12 \u795e\u7d4c\u7ba1\u306e\u5f62\u6210\u904e\u7a0b\u306b\u304a\u3051\u308b\u7d30\u80de\u306e\u5f62\u614b\u5f62\u6210\" width=\"300\" height=\"201\" srcset=\"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2019\/07\/Fig2_600dpi-300x201.jpg 300w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2019\/07\/Fig2_600dpi-768x515.jpg 768w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2019\/07\/Fig2_600dpi-1024x687.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-3228\" class=\"wp-caption-text\">\u56f3\uff12 \u795e\u7d4c\u7ba1\u306e\u5f62\u6210\u904e\u7a0b\u306b\u304a\u3051\u308b\u7d30\u80de\u306e\u5f62\u614b\u5f62\u6210<br>Suzuki et al., Dev. Growth Differ., 54, 266-276, 2012\u3088\u308a<\/p><\/div>\n<p><span style=\"font-size: inherit;\">\u3053\u306e\u7814\u7a76\u306f\u3001\u795e\u7d4c\u7ba1\u5f62\u6210\u304c\u6b63\u5e38\u306b\u8d77\u3053\u3089\u306a\u3044\u30d2\u30c8\u306e\u5148\u5929\u7570\u5e38\uff08\u795e\u7d4c\u7ba1\u9589\u9396\u969c\u5bb3\uff09\u3084\u69d8\u3005\u306a\u4e0a\u76ae\u5f62\u614b\u5f62\u6210\u7570\u5e38\u3092\u4f34\u3046\u75be\u60a3\u306e\u539f\u56e0\u89e3\u660e\u306b\u3082\u3064\u306a\u304c\u308b\u7814\u7a76\u3067\u3059\u3002<\/span><\/p>\n<h3 id=\"bioresource\">2. \u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u4e8b\u696d<\/h3>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1025 alignright\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2017\/05\/63011c1c9514f20c41d9e8206afd8687.jpg\" alt=\"\" width=\"375\" height=\"158\">\u3000\u73fe\u5728\u3001\u30e2\u30c7\u30eb\u4e21\u751f\u985e\u3068\u3057\u3066\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u306b\u6c4e\u7528\u3055\u308c\u3066\u3044\u308b\u30c4\u30e1\u30ac\u30a8\u30eb\u306b\u306f\u3001\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\uff08<em>Xenopus laevis<\/em>\uff09\u3068\u30cd\u30c3\u30bf\u30a4\u30c4\u30e1\u30ac\u30a8\u30eb\uff08<em>Xenopus tropicalis<\/em>\uff09\u304c\u3042\u308a\u307e\u3059\u3002\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u306f\u53e4\u304f\u304b\u3089\u7814\u7a76\u306b\u7528\u3044\u3089\u308c\u3066\u304d\u305f\u7a2e\u3067\u3001\u3068\u3066\u3082\u4e08\u592b\u3067\u98fc\u80b2\u304c\u5bb9\u6613\u306a\u3046\u3048\u3001\u5375\u3084\u80da\u304c\u5927\u304d\u304f\u30de\u30a4\u30af\u30ed\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u3084\u7d44\u7e54\u79fb\u690d\u7b49\u306e\u5b9f\u9a13\u3092\u7c21\u5358\u306b\u304a\u3053\u306a\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cd\u30b7\u30b9\u306b\u3064\u3044\u3066\u3082\u3001\u7cbe\u5b50\u6838\u79fb\u690d\u6cd5\u3084I-SceI\u30e1\u30ac\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6cd5\u3092\u7528\u3044\u308c\u3070\u3001\uff11\u65e5\u306b200-300\u500b\u4f53\u306e\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u3092\u5bb9\u6613\u306b\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u91ce\u751f\u578b\u8fd1\u4ea4\u7cfb\u3082\u3001\u65b0\u6f5f\u5927\u5b66\u306e\u4e95\u7b52\u3086\u307f\u51c6\u6559\u6388\u3089\u304c\u4f5c\u51fa\u3057\u305fJ\u7cfb\u7d71\u3068\u3088\u3070\u308c\u308b\u3082\u306e\u304c\u3042\u308a\u3001\u5168\u30b2\u30ce\u30e0\u914d\u5217\u306e\u89e3\u8aad\u306b\u7528\u3044\u3089\u308c\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u4e16\u4ee3\u6642\u9593\u304c\uff11\u5e74\u4ee5\u4e0a\u3068\u9577\u304f\u3001\u56db\u500d\u4f53\u30b2\u30ce\u30e0\u3092\u3082\u3064\u70ba\u3001\u907a\u4f1d\u5b66\u7684\u7814\u7a76\u306b\u306f\u4f7f\u3044\u3065\u3089\u3044\u3068\u3044\u3046\u5f31\u70b9\u3082\u3042\u308a\u307e\u3059\u3002<br>\u4e00\u65b9\u3001\u30cd\u30c3\u30bf\u30a4\u30c4\u30e1\u30ac\u30a8\u30eb\u306f\u4e16\u4ee3\u6642\u9593\u304c\uff14\u2212\uff16\u30f6\u6708\u3068\u77ed\u304f\u3001\uff12\u500d\u4f53\u306e\u5c0f\u3055\u306a\u30b2\u30ce\u30e0\u3092\u3082\u3064\u70ba\u3001\u907a\u4f1d\u5b66\u7684\u7814\u7a76\u306b\u9069\u3057\u3066\u3044\u307e\u3059\u3002\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cd\u30b7\u30b9\u306b\u3064\u3044\u3066\u306f\u3001I-SceI\u30e1\u30ac\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6cd5\u306b\u3088\u308a\u3001\uff11\u65e5\u306b200-300\u500b\u4f53\u306e\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u3092\u5bb9\u6613\u306b\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5168\u30b2\u30ce\u30e0\u914d\u5217\u3082\u89e3\u8aad\u3055\u308c\u3066\u304a\u308a\u3001\u907a\u4f1d\u5b50\u7834\u58ca\u306fCRISPR\/Cas9\u6cd5\u7b49\u306b\u3088\u308a\u7c21\u5358\u306b\u304a\u3053\u306a\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u98fc\u80b2\u306b\u306f\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u3088\u308a\u53b3\u3057\u3044\u6c34\u8cea\u7ba1\u7406\u304c\u5fc5\u8981\u3067\u3059\u3002\u307e\u305f\u5375\u3084\u80da\u304c\u5c0f\u3055\u3044\u70ba\uff08\u4f53\u7a4d\u3067\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u306e1\/3\u3050\u3089\u3044\uff09\u3001\u30de\u30a4\u30af\u30ed\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u7b49\u306e\u5b9f\u9a13\u306b\u306f\u591a\u5c11\u306e\u6163\u308c\u304c\u5fc5\u8981\u3067\u3059\u3002<br>\u30dd\u30b9\u30c8\u30b2\u30ce\u30e0\u6642\u4ee3\u3092\u8fce\u3048\u305f\u73fe\u5728\u3001\u30c4\u30e1\u30ac\u30a8\u30eb\u3092\u7528\u3044\u305f\u767a\u751f\u3084\u518d\u751f\u3001\u9032\u5316\u306e\u7814\u7a76\u306b\u306f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u3088\u308b\u907a\u4f1d\u5b50\u7834\u58ca\u3068\u3001\u305d\u306e\u7d50\u679c\u89e3\u6790\u306e\u65b9\u6cd5\u3068\u3057\u3066\u3001\u751f\u4f53\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u89e3\u6790\u3084\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30c8\u30fc\u30e0\u89e3\u6790\u7b49\u3092\u6709\u52b9\u306b\u7528\u3044\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u3053\u308c\u3089\u30c4\u30e1\u30ac\u30a8\u30eb\u4ee5\u5916\u306b\u3082\u3001\u30a2\u30db\u30ed\u30fc\u30c8\u30eb\u306f\u30e2\u30c7\u30eb\u4e21\u751f\u985e\u3068\u3057\u3066\u6c4e\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u89e3\u6790\u304c\u666e\u53ca\u3057\u305f\u4eca\u3001\u69d8\u3005\u306a\u5728\u6765\u7a2e\u306e\u30ab\u30a8\u30eb\u3084\u30a4\u30e2\u30ea\u304c\u65b0\u3057\u3044\u5b9f\u9a13\u52d5\u7269\u3084\u907a\u4f1d\u5b50\u8cc7\u6e90\u3068\u3057\u3066\u91cd\u8981\u306b\u306a\u308a\u3064\u3064\u3042\u308a\u307e\u3059\u3002\u79c1\u9054\u306e\u7814\u7a76\u5ba4\u3067\u306f\u3001\u7814\u7a76\u30b3\u30df\u30e5\u30cb\u30c6\u30a3\u306e\u652f\u63f4\u3092\u76ee\u7684\u3068\u3057\u3066\u3001\u3053\u308c\u3089\u306e\u4e21\u751f\u985e\u3092\u6271\u3046\u4ee5\u4e0b\u306e\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u4e8b\u696d\u3092\u5b9f\u65bd\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h5 id=\"nbrp\">1) \u30cd\u30c3\u30bf\u30a4\u30c4\u30e1\u30ac\u30a8\u30eb\u30fb\u30ca\u30b7\u30e7\u30ca\u30eb\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\uff08NBRP\uff09<\/h5>\n<p>AMED\uff08\u65e5\u672c\u533b\u7642\u7814\u7a76\u958b\u767a\u6a5f\u69cb\uff09\u306e\u652f\u63f4\u4e8b\u696d\u3068\u3057\u3066\u3001\u4e3b\u306b\uff14\u7a2e\u985e\u306e\u30cd\u30c3\u30bf\u30a4\u30c4\u30e1\u30ac\u30a8\u30eb\u91ce\u751f\u578b\u8fd1\u4ea4\u7cfb\uff08Nigerian A, Nigerian H, Golden, Ivory Coast\uff09\u306e\u6539\u826f\u30fb\u7dad\u6301\u30fb\u914d\u5e03\u3001\u53ca\u3073\u305d\u308c\u3089\u3092\u7528\u3044\u3066\u4f5c\u88fd\u3055\u308c\u305f\u7d44\u63db\u3048\u4f53\u306e\u53ce\u96c6\u30fb\u7dad\u6301\u30fb\u914d\u5e03\u3092\u304a\u3053\u306a\u3063\u3066\u3044\u307e\u3059\u3002Nigerian A\u306f\u3001\u5168\u30b2\u30ce\u30e0\u914d\u5217\u89e3\u8aad\u306b\u7528\u3044\u3089\u308c\u305f\u500b\u4f53\u306e\u76f4\u7cfb\u7cfb\u7d71\u3067\u3059\u3002Nigerian H\u306fNigerian A\u306b\u8fd1\u3044\u6d3e\u751f\u7cfb\u7d71\u3067\u3001Nigerian A\u3088\u308a\u7523\u5375\u6570\u304c\u591a\u3044\u50be\u5411\u304c\u3042\u308a\u307e\u3059\u3002Golden\u306fNigerian A\u306b\u5bfe\u3057\u3066Nigerian H\u3088\u308a\u907a\u4f1d\u7684\u306b\u96e2\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u98fc\u80b2\u304c\u5bb9\u6613\u3067\u4f7f\u3044\u6613\u3044\u7cfb\u7d71\u3067\u3059\u3002Ivory Coast\u306fNigerian A\u304b\u3089\u6700\u3082\u907a\u4f1d\u7684\u306b\u96e2\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u53d7\u7cbe\u7387\u304c\u5b89\u5b9a\u3057\u3066\u9ad8\u304f\u4f7f\u3044\u6613\u3044\u7cfb\u7d71\u3067\u3059\u3002\u7d44\u63db\u3048\u4f53\u3068\u3057\u3066\u306f\u3001\u7279\u5b9a\u306e\u7d44\u7e54\u3084\u5668\u5b98\u3001\u4f8b\u3048\u3070\u8133\u795e\u7d4c\u7cfb\u3084\u8840\u7ba1\u7cfb\u3092GFP\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u6a19\u8b58\u3057\u305f\u3088\u3046\u306a\u751f\u4f53\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u89e3\u6790\u7528\u306e\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u7cfb\u7d71\u3084\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u3088\u3063\u3066\u4f5c\u88fd\u3055\u308c\u305f\u6c4e\u7528\u6027\u306e\u3042\u308b\u5909\u7570\u4f53\uff08\u75be\u60a3\u30e2\u30c7\u30eb\u7b49\uff09\u306e\u53ce\u96c6\u30fb\u7dad\u6301\u30fb\u914d\u5e03\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002\u8a73\u3057\u304f\u306f\u672c\u30bb\u30f3\u30bf\u30fcHP\u306e\u30c8\u30c3\u30d7\u306b\u63b2\u8f09\u3055\u308c\u305f\u30ea\u30f3\u30af\u3088\u308a\u3001\u30cd\u30c3\u30bf\u30a4\u30c4\u30e1\u30ac\u30a8\u30ebNBRP\u306e\u30a6\u30a7\u30d6\u30b5\u30a4\u30c8\u3092\u3054\u89a7\u4e0b\u3055\u3044\u3002<\/p>\n<h5 id=\"keitou\">2) \u305d\u306e\u4ed6\u306e\u30e2\u30c7\u30eb\u4e21\u751f\u985e\u3084\u5728\u6765\u7a2e\u306e\u30ea\u30bd\u30fc\u30b9\u4e8b\u696d\uff08\u7cfb\u7d71\u7dad\u6301\u73ed\uff09<\/h5>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1026 alignright\" src=\"https:\/\/amphibian.hiroshima-u.ac.jp\/oginolab\/wp-content\/uploads\/2017\/05\/479fcd0e78db7716155cd8279b6c0eaf.jpg\" alt=\"\" width=\"227\" height=\"170\" srcset=\"https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/479fcd0e78db7716155cd8279b6c0eaf.jpg 328w, https:\/\/amphibian.hiroshima-u.ac.jp\/~oginolab\/wp-content\/uploads\/2017\/05\/479fcd0e78db7716155cd8279b6c0eaf-300x225.jpg 300w\" sizes=\"auto, (max-width: 227px) 100vw, 227px\" \/>\u3000\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u306b\u3064\u3044\u3066\u306f\u3001\u91ce\u751f\u578b\u8fd1\u4ea4\u7cfb\uff08J\u7cfb\u7d71\uff09\u3084\u3001\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7cfb\u306e\u963b\u5bb3\u56e0\u5b50\u3092\u767a\u73fe\u3059\u308b\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u7cfb\u7d71\u3001\u751f\u4f53\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u89e3\u6790\u7528\u306b\u7279\u5b9a\u306e\u7d44\u7e54\u3067GFP\u3092\u767a\u73fe\u3059\u308b\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u7cfb\u7d71\u7b49\u306e\u958b\u767a\u30fb\u53ce\u96c6\u30fb\u7dad\u6301\u30fb\u914d\u5e03\u3092\u304a\u3053\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u30a2\u30db\u30ed\u30fc\u30c8\u30eb\u306b\u95a2\u3057\u3066\u306f\u3001\u91ce\u751f\u578b\u3084\u30a2\u30eb\u30d3\u30ce\u306e\u8fd1\u4ea4\u7cfb\u306e\u53ce\u96c6\u30fb\u7dad\u6301\u30fb\u914d\u5e03\u3092\u304a\u3053\u306a\u3063\u3066\u3044\u307e\u3059\uff08\u73fe\u5728\u3001\u63d0\u4f9b\u53ef\u80fd\u306a\u7cfb\u7d71\u306e\u30ea\u30b9\u30c8\u3092\u6e96\u5099\u4e2d\u3067\u3059\u3002\uff09\u3002<\/p>\n<h3 id=\"ob\">3. 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